Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies.
Nano Lett
; 14(8): 4197-204, 2014 Aug 13.
Article
in En
| MEDLINE
| ID: mdl-24598026
ABSTRACT
We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanostructures
/
Membranes, Artificial
/
Metaphase
Limits:
Humans
Language:
En
Journal:
Nano Lett
Year:
2014
Document type:
Article
Affiliation country:
Germany