Confocal analysis of the exopolysaccharide matrix of Candida albicans biofilms.
J Investig Clin Dent
; 6(3): 179-85, 2015 Aug.
Article
in En
| MEDLINE
| ID: mdl-24610643
ABSTRACT
AIM:
Confocal laser-scanning microscopy (CLSM) was carried out to investigate the exopolysaccharide matrix of Candida albicans (C. albicans) biofilms developed on denture material under dietary carbohydrate exposure.METHODS:
Biofilms were developed on poly(methyl methacrylate) discs in culture media without (control) or with supplementation by glucose or sucrose for 72 h. For the CLSM analysis, biofilms were labeled with concanavalin A (ConA) during its development. Afterwards, biofilms were also labeled with SYTO-9. To confirm the results, the matrix was investigated by the phenol-sulfuric method. Data were analyzed by anova, followed by Tukey's test, with the level of significance set at 5%.RESULTS:
The use of ConA during biofilm development provided effective labeling of the exopolysaccharide matrix. The exposure to sucrose resulted in biofilms with the highest exopolysaccharide matrix biovolume (P < 0.05). The characterization obtained by CLSM was confirmed by the phenol-sulfuric method.CONCLUSION:
Confocal laser-scanning microscopy was found to be an effective tool for investigating the exopolysaccharide matrix of C. albicans biofilms, and exposure to sucrose resulted in increased matrix production.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candida albicans
/
Biofilms
/
Fungal Polysaccharides
Type of study:
Clinical_trials
Limits:
Humans
Language:
En
Journal:
J Investig Clin Dent
Year:
2015
Document type:
Article
Affiliation country:
Brazil