[Effect of Shengji Huayu Recipe on the expression of MMP-3 and TIMP-1 in skin ulcer tissue of diabetic rats].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 34(2): 218-23, 2014 Feb.
Article
in Zh
| MEDLINE
| ID: mdl-24672949
ABSTRACT
OBJECTIVE:
To study the effect of Shengji Huayu Recipe (SHR)on the expression of MMP-3 and TIMP-1 in the skin ulcer tissue of diabetic rats.METHODS:
The skin ulcer model was established in diabetic mice. Different compatibility proportions of SHR [the ratio of Shengji Recipe (SJR) to Huayu Recipe (HYR) = 21, 11, and 12, respectively] were used to intervene. The expression of MMP-3 protein in the skin ulcer of diabetic rats was detected by Western blot method,and TIMP-1 protein was detected by immunohistochemical assay.RESULTS:
At each time point, there was no statistical difference in the blood glucose level among groups (P > 0.05). But all of them increased significantly,when compared with those of the normal wound group (P < 0.01). As for the difference between after would area treatment and before would area treatment, better effect was obtained in the SHR No. 3 group and the normal ulcer group than in the diabetic ulcer model group (P < 0.05). Results of Western blot showed that the MMP-3 protein expression was higher in the SHR No. 2 group than in the SHR No.3 group (P < 0.05). Immunohistochemical results showed that TIMP-1 protein expression was lower in the SHR No. 2 group than in the SHR No. 3 group and the diabetic ulcer model group (P < 0.05). TIMP-1 protein expression was higherin the SHR No. 3 group than in the SHR No. 2 group (P < 0.01).CONCLUSION:
Using SHR No.3 was conducive to the promotion of wound healing in early wound repair stage, and using SHR No. 2 might be conducive to inhibiting the formation of pathological scar.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin Ulcer
/
Drugs, Chinese Herbal
/
Matrix Metalloproteinase 3
/
Tissue Inhibitor of Metalloproteinase-1
/
Diabetes Mellitus, Experimental
Limits:
Animals
Language:
Zh
Journal:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2014
Document type:
Article