A high-throughput colorimetric screening assay for terpene synthase activity based on substrate consumption.

Furubayashi, Maiko; Ikezumi, Mayu; Kajiwara, Jun; Iwasaki, Miki; Fujii, Akira; Li, Ling; Saito, Kyoichi; Umeno, Daisuke

Furubayashi, Maiko; Ikezumi, Mayu; Kajiwara, Jun; Iwasaki, Miki; Fujii, Akira; Li, Ling; Saito, Kyoichi; Umeno, Daisuke.

Affiliation

Furubayashi M; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Ikezumi M; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Kajiwara J; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Iwasaki M; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Fujii A; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Li L; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Saito K; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan.
Umeno D; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, Japan.

PLoS One ; 9(3): e93317, 2014.

Article in En | MEDLINE | ID: mdl-24681801

Subject(s)

Alkyl and Aryl Transferases/chemistry; Colorimetry/methods; High-Throughput Screening Assays/methods; Amino Acid Sequence; Carotenoids/chemistry; Escherichia coli/chemistry; Molecular Sequence Data; Substrate Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colorimetry / Alkyl and Aryl Transferases / High-Throughput Screening Assays Type of study: Diagnostic_studies / Screening_studies Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2014 Document type: Article Affiliation country: Japan

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