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Isolation and characterization of a ranavirus from koi, Cyprinus carpio L., experiencing mass mortalities in India.
George, M R; John, K R; Mansoor, M M; Saravanakumar, R; Sundar, P; Pradeep, V.
Affiliation
  • George MR; Department of Aquaculture, Fisheries College and Research Institute, Tuticorin, India.
J Fish Dis ; 38(4): 389-403, 2015 Apr.
Article in En | MEDLINE | ID: mdl-24720625
ABSTRACT
We investigated mass mortalities of koi, Cyprinus carpio Linnaeus, 1758, experienced in South Indian fish farms by virus isolation, electron microscopy, PCR detection, sequencing of capsid protein gene and transmission studies. Samples of moribund koi brought to the laboratory suffered continuous mortality exhibiting swimming abnormalities, intermittent surfacing and skin darkening. Irido-like virus was isolated from the infected fish in the indigenous snakehead kidney cell line (SNKD2a). Icosahedral virus particles of 100 to 120 nm were observed in the infected cell cultures, budding from the cell membrane. Virus transmission and pathogenicity studies revealed that horizontal transmission occurred associated with mortality. PCR analysis of infected fish and cell cultures confirmed the presence of Ranavirus capsid protein sequences. Sequence analysis of the major capsid protein gene showed an identity of 99.9% to that of largemouth bass virus isolated from North America. Detection and successful isolation of this viral agent becomes the first record of isolation of a virus resembling Santee-Cooper Ranavirus from a koi and from India. We propose the name koi ranavirus to this agent.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Virus Infections / Fish Diseases Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: J Fish Dis Journal subject: BIOLOGIA / MEDICINA VETERINARIA Year: 2015 Document type: Article Affiliation country: India

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Virus Infections / Fish Diseases Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: J Fish Dis Journal subject: BIOLOGIA / MEDICINA VETERINARIA Year: 2015 Document type: Article Affiliation country: India