Selective identification and quantification of saccharin by liquid chromatography and fluorescence detection.
Food Chem
; 159: 309-15, 2014 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-24767060
ABSTRACT
High-pressure liquid chromatography with ultra-violet detection (HPLC-UV) is one of the most commonly used methods to identify and quantify saccharin in non-alcoholic beverages. However, due to the wide variety of interfering UV spectra in saccharin-containing beverage matrices, the same method cannot be used to measure this analyte accurately. We have developed a new, highly effective method to identify and quantify saccharin using HPLC with fluorescence detection (HPLC-FLD). The excitation wavelength (250 nm) and emission wavelength (440 nm) chosen increased selectivity for all matrices and ensured few changes were required in the mobile phase or other parameters. The presence of saccharin in non-diet beverages - a fraud commonly used to replace more expensive sucrose - was confirmed by comparing coincident peaks as well as the emission spectra of standards and samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharin
/
Beverages
/
Chromatography, High Pressure Liquid
/
Food Additives
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
Food Chem
Year:
2014
Document type:
Article