New astrovirus in human feces from Burkina Faso.
J Clin Virol
; 60(2): 161-4, 2014 Jun.
Article
in En
| MEDLINE
| ID: mdl-24775733
ABSTRACT
BACKGROUND:
A significant fraction of cases of diarrhea, a leading cause of childhood mortality worldwide, remain unexplained.OBJECTIVES:
To identify viruses in unexplained cases of diarrhea using an unbiased metagenomics approach. STUDYDESIGN:
Viral nucleic acids were enriched from the feces from 48 cases of unexplained diarrhea from Burkina Faso, sequenced, and compared against all known viral genomes.RESULTS:
The full genome of a highly divergent astrovirus was sequenced in a sample co-infected with parechovirus 1. RT-PCR identified a single astrovirus infection in these 48 patients indicating a low prevalence. Human astrovirus-BF34 was most closely related to mamastrovirus species 8 and 9 also found in human with which it shared 62%, 74%, and 57% amino acid identities over its protease, RNA dependent RNA polymerase and capsid proteins, respectively.CONCLUSIONS:
Burkina Faso astrovirus is proposed as prototype for a novel species in the genus Mamastrovirus, here tentatively called Mamastrovirus 20, representing the fifth human astrovirus species.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mamastrovirus
/
Sequence Analysis, DNA
/
Astroviridae Infections
/
Diarrhea
Type of study:
Risk_factors_studies
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Country/Region as subject:
Africa
Language:
En
Journal:
J Clin Virol
Journal subject:
VIROLOGIA
Year:
2014
Document type:
Article
Affiliation country:
United States