Optimization of single-cell electroporation protocol for forced gene expression in primary neuronal cultures.

Nishikawa, Shin; Hirashima, Naohide; Tanaka, Masahiko

Nishikawa, Shin; Hirashima, Naohide; Tanaka, Masahiko.

Affiliation

Nishikawa S; Department of Cellular Biophysics, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 467-8603, Japan.

Mol Biotechnol ; 56(9): 824-32, 2014 Sep.

Article in En | MEDLINE | ID: mdl-24794046

Subject(s)

Electroporation/methods; Green Fluorescent Proteins/biosynthesis; Purkinje Cells/cytology; Single-Cell Analysis/methods; Animals; Cell Culture Techniques/methods; Cells, Cultured; Gene Expression; Green Fluorescent Proteins/genetics; Mice; Mice, Inbred C57BL; Plasmids/genetics; Plasmids/metabolism; Purkinje Cells/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Purkinje Cells / Electroporation / Green Fluorescent Proteins / Single-Cell Analysis Limits: Animals Language: En Journal: Mol Biotechnol Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2014 Document type: Article Affiliation country: Japan Country of publication: Switzerland

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