Generation of homologous cell pairs using the oral lymphatic system.
Int J Clin Exp Pathol
; 7(4): 1563-71, 2014.
Article
in En
| MEDLINE
| ID: mdl-24817952
ABSTRACT
The purpose of this study is to establish in vivo and in vitro models for studying lymphatic metastasis of squamous cell carcinoma (SCC). Three cell lines CAL-27, Tca-83, and HeLa were injected into the tongue of nude mice. Forty days after injection, we could isolate cells of 2 homologous cell lines LN-CAL-27 and LN-HeLa from lymph node metastasis lesions. Then, the homologous cell pairs were compared by the CCK-8 assay, wound healing assay, real-time PCR, western blot, and animal experiments. The results showed that all the three cell lines could be used to establish lymphatic metastasis animal models, and the lymphatic metastasis process was observed clearly. In addition, the homologous cell pairs performed differently from parent lines with respect to biological behavior and lymphatic metastasis-related gene and protein expression. In conclusion, CAL-27, Tca-83, and HeLa cells could be used to simulate the lymphatic metastasis process of oral cancer in vivo. Furthermore, the homologous cell pairs (CAL-27 and LN-CAL-27; HeLa and LN-HeLa) are potential tools for in vitro investigation of the mechanisms underlying metastasis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tongue Neoplasms
/
Carcinoma, Squamous Cell
/
Uterine Cervical Neoplasms
/
Lymphatic Metastasis
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Int J Clin Exp Pathol
Journal subject:
PATOLOGIA
Year:
2014
Document type:
Article
Affiliation country:
China