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Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats.
Maganhin, Carla C; Simões, Ricardo S; Fuchs, Luiz F P; Sasso, Gisela R S; Simões, Manuel J; Baracat, Edmund C; Soares, Jose M.
Affiliation
  • Maganhin CC; Department of Gynecology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, Brazil. Electronic address: kmasouza@hotmail.com.
  • Simões RS; Department of Obstetrics and Gynecology, São Paulo School of Medicine, University São Paulo, São Paulo, Brazil.
  • Fuchs LF; Department of Obstetrics and Gynecology, São Paulo School of Medicine, University São Paulo, São Paulo, Brazil.
  • Sasso GR; Department of Morphology and Genetics Paulista School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • Simões MJ; Department of Morphology and Genetics Paulista School of Medicine, Federal University of São Paulo, São Paulo, Brazil.
  • Baracat EC; Department of Obstetrics and Gynecology, São Paulo School of Medicine, University São Paulo, São Paulo, Brazil.
  • Soares JM; Department of Obstetrics and Gynecology, São Paulo School of Medicine, University São Paulo, São Paulo, Brazil.
Fertil Steril ; 102(1): 291-8, 2014 Jul.
Article in En | MEDLINE | ID: mdl-24825418
OBJECTIVE: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. DESIGN: Experimental research. SETTING: University research laboratory. ANIMAL(S): Thirty female adult rats. INTERVENTION(S): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 µg/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. MAIN OUTCOME MEASURE(S): Biologic molecular study followed by immunohistochemical analysis. RESULT(S): The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. CONCLUSION(S): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ovary / Pineal Gland / Progesterone / Steroid Hydroxylases / Hormone Replacement Therapy / Estrogens / Melatonin Limits: Animals Language: En Journal: Fertil Steril Year: 2014 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ovary / Pineal Gland / Progesterone / Steroid Hydroxylases / Hormone Replacement Therapy / Estrogens / Melatonin Limits: Animals Language: En Journal: Fertil Steril Year: 2014 Document type: Article Country of publication: United States