Mechanical properties of mouse lungs along organ decellularization by sodium dodecyl sulfate.
Respir Physiol Neurobiol
; 200: 1-5, 2014 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-24837837
ABSTRACT
Lung decellularization is based on the use of physical, chemical, or enzymatic methods to break down the integrity of the cells followed by a treatment to extract the cellular material from the lung scaffold. The aim of this study was to characterize the mechanical changes throughout the different steps of lung decellularization process. Four lungs from mice (C57BL/6) were decellularized by using a conventional protocol based on sodium dodecyl sulfate. Lungs resistance (R(L)) and elastance (E(L)) were measured along decellularization steps and were computed by linear regression fitting of tracheal pressure, flow, and volume during mechanical ventilation. Transients differences found were more distinct in an intermediate step after the lungs were rinsed with deionized water and treated with 1% SDS, whereupon the percentage of variation reached approximately 80% for resistance values and 30% for elastance values. In conclusion, although a variation in extracellular matrix stiffness was observed during the decellularization process, this variation can be considered negligible overall because the resistance and elastance returned to basal values at the final decellularization step.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sodium Dodecyl Sulfate
/
Respiratory Mechanics
/
Detergents
/
Extracellular Matrix
/
Lung
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
Respir Physiol Neurobiol
Year:
2014
Document type:
Article
Affiliation country:
Brazil