The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification.
Nat Cell Biol
; 16(6): 516-28, 2014 Jun.
Article
in En
| MEDLINE
| ID: mdl-24859004
ABSTRACT
The precise relationship of embryonic stem cells (ESCs) to cells in the mouse embryo remains controversial. We present transcriptional and functional data to identify the embryonic counterpart of ESCs. Marker profiling shows that ESCs are distinct from early inner cell mass (ICM) and closely resemble pre-implantation epiblast. A characteristic feature of mouse ESCs is propagation without ERK signalling. Single-cell culture reveals that cell-autonomous capacity to thrive when the ERK pathway is inhibited arises late during blastocyst development and is lost after implantation. The frequency of deriving clonal ESC lines suggests that all E4.5 epiblast cells can become ESCs. We further show that ICM cells from early blastocysts can progress to ERK independence if provided with a specific laminin substrate. These findings suggest that formation of the epiblast coincides with competence for ERK-independent self-renewal in vitro and consequent propagation as ESC lines.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Cell Differentiation
/
Cell Lineage
/
Pluripotent Stem Cells
/
Cell Proliferation
/
Embryonic Stem Cells
/
Blastocyst Inner Cell Mass
/
Germ Layers
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Nat Cell Biol
Year:
2014
Document type:
Article
Affiliation country:
United kingdom