Isoprenoid phosphonophosphates as glycosyltransferase acceptor substrates.
J Am Chem Soc
; 136(24): 8492-5, 2014 Jun 18.
Article
in En
| MEDLINE
| ID: mdl-24866828
ABSTRACT
Glycosyltransferases that act on polyprenol pyrophosphate substrates are challenging to study because their lipid-linked substrates are difficult to isolate from natural sources and arduous to synthesize. To facilitate access to glycosyl acceptors, we assembled phosphonophosphate analogues and showed these are effective substrate surrogates for GlfT1, the essential product of mycobacterial gene Rv3782. Under chemically defined conditions, the galactofuranosyltransferase GlfT1 catalyzes the formation of a tetrasaccharide sequence en route to assembly of the mycobacterial galactan.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyisoprenyl Phosphates
/
Glycosyltransferases
Language:
En
Journal:
J Am Chem Soc
Year:
2014
Document type:
Article
Affiliation country:
United States