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Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of Nelumbo nucifera Gaertn. (lotus), an aquatic edible plant.
Mahmad, Noraini; Taha, Rosna Mat; Othman, Rashidi; Saleh, Azani; Hasbullah, Nor Azlina; Elias, Hashimah.
Affiliation
  • Mahmad N; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • Taha RM; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  • Othman R; International Institute for Halal Research and Training (INHART), Herbarium Unit, Department of Landscape Architecture (KAED), International Islamic University Malaysia, 53100 Kuala Lumpur, Malaysia.
  • Saleh A; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Faculty of Applied Science, MARA University of Technology, 40450 Shah Alam, Selangor, Malaysia.
  • Hasbullah NA; Department of Agriculture Sciences, Faculty of Technical and Vocational Education, Sultan Idris Education University, 35900 Tanjung Malim, Perak, Malaysia.
  • Elias H; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
ScientificWorldJournal ; 2014: 745148, 2014.
Article in En | MEDLINE | ID: mdl-24895660
In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Growth Regulators / Plants, Edible / Acetic Acid / Nelumbo / Naphthols Language: En Journal: ScientificWorldJournal Journal subject: MEDICINA Year: 2014 Document type: Article Affiliation country: Malaysia Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Growth Regulators / Plants, Edible / Acetic Acid / Nelumbo / Naphthols Language: En Journal: ScientificWorldJournal Journal subject: MEDICINA Year: 2014 Document type: Article Affiliation country: Malaysia Country of publication: United States