Development and validation of a quantitative real-time polymerase chain reaction assay specific for the detection of Rickettsia felis and not Rickettsia felis-like organisms.

Odhiambo, Antony M; Maina, Alice N; Taylor, Melissa L; Jiang, Ju; Richards, Allen L

Odhiambo, Antony M; Maina, Alice N; Taylor, Melissa L; Jiang, Ju; Richards, Allen L.

Affiliation

Odhiambo AM; Viral and Rickettsial Diseases Department, Naval Medical Research Center , Silver Spring, Maryland.

Vector Borne Zoonotic Dis ; 14(7): 476-81, 2014 Jul.

Article in En | MEDLINE | ID: mdl-24918658

Subject(s)

Arthropod Vectors/microbiology; Real-Time Polymerase Chain Reaction/methods; Rickettsia Infections/diagnosis; Rickettsia felis/isolation & purification; Animals; Bacterial Proteins/genetics; Base Sequence; DNA Primers/genetics; DNA, Bacterial/genetics; Humans; Limit of Detection; Molecular Sequence Data; Rickettsia Infections/microbiology; Rickettsia felis/genetics; Risk Assessment; Sequence Alignment; Time Factors

Key words

Diagnostics; Rickettsia felis; Risk assessment; Vector borne; qPCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arthropod Vectors / Rickettsia Infections / Rickettsia felis / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Etiology_studies / Prognostic_studies / Risk_factors_studies Limits: Animals / Humans Language: En Journal: Vector Borne Zoonotic Dis Journal subject: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Year: 2014 Document type: Article Country of publication: United States

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