In vitro activation of NAD-dependent alcohol dehydrogenases by Nudix hydrolases is more widespread than assumed.
FEBS Lett
; 588(17): 2993-9, 2014 Aug 25.
Article
in En
| MEDLINE
| ID: mdl-24928437
ABSTRACT
In the Gram-positive methylotroph Bacillus methanolicus, methanol oxidation is catalyzed by an NAD-dependent methanol dehydrogenase (Mdh) that belongs to the type III alcohol dehydrogenase (Adh) family. It was previously shown that the in vitro activity of B. methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase. Here we show that this feature is not unique, but more widespread among type III Adhs in combination with Act or other Act-like Nudix hydrolases. In addition, we studied the effect of site directed mutations in the predicted active site of Mdh and two other type III Adhs with regard to activity and activation by Act.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrophosphatases
/
Bacteria
/
Alcohol Oxidoreductases
/
NAD
Type of study:
Prognostic_studies
Language:
En
Journal:
FEBS Lett
Year:
2014
Document type:
Article
Affiliation country:
Switzerland