Use of tandem affinity chromatography for purification of cannabinoid receptor CB2.
Methods Mol Biol
; 1177: 107-20, 2014.
Article
in En
| MEDLINE
| ID: mdl-24943318
ABSTRACT
Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion that lack either N- or C-terminal tags. Here, we describe the use of two small affinity tags, a poly-histidine tag and a Strep-tag for expression and purification of the human cannabinoid receptor CB2, an integral membrane G protein-coupled receptor.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Affinity
/
Receptor, Cannabinoid, CB2
/
Molecular Biology
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2014
Document type:
Article
Affiliation country:
United States
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA