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Effects of cigarette smoke extract on human airway smooth muscle cells in COPD.
Chen, Ling; Ge, Qi; Tjin, Gavin; Alkhouri, Hatem; Deng, Linghong; Brandsma, Corry-Anke; Adcock, Ian; Timens, Wim; Postma, Dirkje; Burgess, Janette K; Black, Judith L; Oliver, Brian G G.
Affiliation
  • Chen L; Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia.
  • Ge Q; Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia.
  • Tjin G; Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Central Clinical School, The University of Sydney, Sydney, NSW, Australia.
  • Alkhouri H; Respiratory Research Group, Faculty of Pharmacy, The University of Sydney, Sydney, NSW, Australia.
  • Deng L; Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Shapingba, Chongqing, China Institute of Biomedical Engineering and Health Science, Changzhou University, Changzhou, Jiangsu, China.
  • Brandsma CA; Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Adcock I; Thoracic Medicine, Imperial College London, National Heart and Lung Institute, London, UK.
  • Timens W; Dept of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Postma D; Dept of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Burgess JK; Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia Central Clinical School, The University of Sydney, Sydney, NSW, Australia.
  • Black JL; Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia Discipline of Pharmacology, The University of Sydney, Sydney, NSW, Australia.
  • Oliver BG; Woolcock Institute of Medical Research, The University of Sydney, Sydney, NSW, Australia School of Medical and Molecular Biosciences, University of Technology Sydney, Sydney, NSW, Australia brian.oliver@uts.edu.au.
Eur Respir J ; 44(3): 634-46, 2014 Sep.
Article in En | MEDLINE | ID: mdl-24969654
ABSTRACT
We hypothesised that the response to cigarette smoke in airway smooth muscle (ASM) cells from smokers with chronic obstructive pulmonary disease (COPD) would be intrinsically different from smokers without COPD, producing greater pro-inflammatory mediators and factors relating to airway remodelling. ASM cells were obtained from smokers with or without COPD, and then stimulated with cigarette smoke extract (CSE) or transforming growth factor-ß1. The production of chemokines and matrix metalloproteinases (MMPs) were measured by ELISA, and the deposition of collagens by extracellular matrix ELISA. The effects of CSE on cell attachment and wound healing were measured by toluidine blue attachment and cell tracker green wound healing assays. CSE increased the release of CXCL8 and CXCL1 from human ASM cells, and cells from smokers with COPD produced more CSE-induced CXCL1. The production of MMP-1, -3 and -10, and the deposition of collagen VIII alpha 1 (COL8A1) were increased by CSE, especially in the COPD group which had higher production of MMP-1 and deposition of COL8A1. CSE decreased ASM cell attachment and wound healing in the COPD group only. ASM cells from smokers with COPD were more sensitive to CSE stimulation, which may explain, in part, why some smokers develop COPD.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bronchi / Smoking / Pulmonary Disease, Chronic Obstructive / Myocytes, Smooth Muscle Limits: Humans Language: En Journal: Eur Respir J Year: 2014 Document type: Article Affiliation country: Australia
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bronchi / Smoking / Pulmonary Disease, Chronic Obstructive / Myocytes, Smooth Muscle Limits: Humans Language: En Journal: Eur Respir J Year: 2014 Document type: Article Affiliation country: Australia