Measuring caspase activity in vivo.
Methods Enzymol
; 544: 251-69, 2014.
Article
in En
| MEDLINE
| ID: mdl-24974293
ABSTRACT
Caspases are a family of integral proteases playing a role in apoptosis. The importance of apoptosis in disease has made these proteases not only an attractive drug target but also a focal point for measuring apoptosis in vivo. The critical role caspases play in determining cell death has led to the development of a wide array of technologies to measure caspase activity in vivo, ranging from small molecule PET imaging reagents to fluorescent and luminescent protein-based reporters used in whole animal and cell-based applications. This chapter reviews this wide range of technologies available as well as the most appropriate applications for each reagent and the mechanism of how it measures caspase activity in vivo.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Caspases
/
Enzyme Activation
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Enzymol
Year:
2014
Document type:
Article
Affiliation country:
United States