T lymphocyte subset modulation of hepatic fibroblast function in murine schistosomiasis.
Am J Trop Med Hyg
; 41(4): 454-9, 1989 Oct.
Article
in En
| MEDLINE
| ID: mdl-2508503
ABSTRACT
We have previously shown that co-culture of T cell enriched spleen lymphocytes can reduce collagen synthesis and stimulate proliferative activity by liver fibroblasts from S. mansoni infected mice. The present study examines which subset of T cells is responsible for this modulation. Co-culture of fibroblasts with the total T cell population lead to significant stimulation in fibroblast proliferative activity and a significant decrease in the incorporation of 14C-proline into collagenase-sensitive protein. There was virtually no effect on total incorporation of 14C-proline in non-collagen proteins. An additional significant increase in fibroblast proliferative activity and another significant decrease in collagen synthesis accompanied by a 2-fold increase in non-collagen protein production was noted when fibroblasts were co-cultured with Lyt-1+ cells. Co-culture of fibroblasts with Lyt-2+ cells did not differ significantly from co-culture with total T cells. Mitomycin treatment of the lymphocytes blunted their specific effects, suggesting that proliferation of T cells is required for maximal exertion of their regulatory activity. These results suggest that the T cells which mediate these effects belong to the Lyt-1+ helper class and are distinct from the Lyt-2+ suppressor cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schistosomiasis
/
T-Lymphocytes
/
Fibroblasts
/
Liver
Limits:
Animals
Language:
En
Journal:
Am J Trop Med Hyg
Year:
1989
Document type:
Article
Affiliation country:
Egypt