[Determination of equilibrium solubility and n-octanol/water partition coefficient of pulchinenosiden D by HPLC].
Zhongguo Zhong Yao Za Zhi
; 39(9): 1593-6, 2014 May.
Article
in Zh
| MEDLINE
| ID: mdl-25095367
ABSTRACT
OBJECTIVE:
To determine the equilibrium solubility of pulchinenosiden D in different solvents and its n-octanol/water partition coefficients.METHOD:
Combining shaking flask method and high performance liquid chromatography (HPLC) to detect the n-octanol/water partition coefficients of pulchinenosiden D, the equilibrium solubility of pulchinenosiden D in six organic solvents and different pH buffer solution were determined by HPLC analysis.RESULT:
n-Octanol/water partition coefficients of pulchinenosiden D in different pH were greater than zero, the equilibrium solubility of pulchinenosiden D was increased with increase the pH of the buffer solution. The maximum equilibrium solubility of pulchinenosiden D was 255.89 g x L(-1) in methanol, and minimum equilibrium solubility of pulchinenosiden D was 0.20 g x L(-1) in acetonitrile.CONCLUSION:
Under gastrointestinal physiological conditions, pulchinenosiden D exists in molecular state and it has good absorption but poor water-solubility, so increasing the dissolution rate of pulchinenosiden D may enhance its bioavailability.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drugs, Chinese Herbal
/
Water
/
Chromatography, High Pressure Liquid
/
1-Octanol
Limits:
Humans
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Journal subject:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Year:
2014
Document type:
Article
Publication country:
CHINA
/
CN
/
REPUBLIC OF CHINA