PCR-based prediction of type I ß-exotoxin production in Bacillus thuringiensis strains.
J Invertebr Pathol
; 122: 28-31, 2014 Oct.
Article
in En
| MEDLINE
| ID: mdl-25132154
ABSTRACT
Some Bacillus thuringiensis strains secrete type I ß-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting ß-exotoxin. With the aim of establish a first rapid approach for prediction of type I ß-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sugar Acids
/
Bacillus thuringiensis
/
Adenosine
/
Polymerase Chain Reaction
Type of study:
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
J Invertebr Pathol
Year:
2014
Document type:
Article
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA