Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols.
Dis Aquat Organ
; 111(1): 15-22, 2014 Aug 21.
Article
in En
| MEDLINE
| ID: mdl-25144113
ABSTRACT
Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 369-23) exceeded the diagnostic sensitivity of 0.85 (95% CI 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reverse Transcriptase Polymerase Chain Reaction
/
Novirhabdovirus
/
Hemorrhagic Septicemia, Viral
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic_studies
/
Guideline
/
Screening_studies
Limits:
Animals
Language:
En
Journal:
Dis Aquat Organ
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Year:
2014
Document type:
Article
Affiliation country:
United States