A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of L-lactic acid.
Biotechnol Lett
; 36(12): 2545-9, 2014 Dec.
Article
in En
| MEDLINE
| ID: mdl-25214213
ABSTRACT
The growth rate and maximum biomass of Bacillus coagulans 2-6 were inhibited by lactate; inhibition by sodium lactate was stronger than by calcium lactate. The differences of protein expressions by B. coagulans 2-6 under the lactate stress were determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under the non-stress condition, calcium lactate stress and sodium lactate stress, the number of detected protein spots was 1,571 ± 117, 1,281 ± 231 and 904 ± 127, respectively. Four proteins with high expression under lactate stress were identified lactate dehydrogenase, cysteine synthase A, aldo/keto reductase and ribosomal protein L7/L12. These proteins are thus potential targets for the reconstruction of B. coagulans to promote its resistance to lactate stress.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacillus
/
Proteome
/
Lactates
Language:
En
Journal:
Biotechnol Lett
Year:
2014
Document type:
Article