Evaluation of the Sebia Minicap Flex Piercing capillary electrophoresis for hemoglobinopathy testing.
Int J Lab Hematol
; 37(3): 420-5, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-25324031
ABSTRACT
INTRODUCTION:
Capillary zone electrophoresis (CZE) at alkaline pH is one of the techniques used for hemoglobinopathy screening. In this study, an evaluation of the performance of a lower throughput CZE instrument, the Sebia Minicap Flex Piercing system, for this purpose is reported for the first time.METHODS:
The analytical performance of the Sebia Minicap Flex Piercing system was evaluated. Furthermore, a method comparison between the Sebia Minicap Flex Piercing and two HPLC methods, that is, the Bio-Rad Variant Classic(™) and the Bio-Rad D-10(™) systems was performed by measuring samples with and without clinically relevant hemoglobin disorders.RESULTS:
The analytical performance was acceptable for the determination of HbA, HbA2, HbS, and HbF, with an imprecision ≤2.0%. Method comparison showed a linear correlation for HbA2, HbF, and HbS measurements. Clinical concordance was acceptable when comparing CZE and HPLC.CONCLUSIONS:
Lower throughput CZE using the Sebia Minicap Flex Piercing can be used for precise and accurate first line screening and follow-up of hemoglobinopathies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Electrophoresis, Capillary
/
Hemoglobinopathies
Limits:
Humans
Language:
En
Journal:
Int J Lab Hematol
Journal subject:
HEMATOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Belgium