Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production in Pichia pastoris by the identification and removal of protease cleavage sites.

Spiegel, Holger; Schinkel, Helga; Kastilan, Robin; Dahm, Pia; Boes, Alexander; Scheuermayer, Matthias; Chudobová, Ivana; Maskus, Dominika; Fendel, Rolf; Schillberg, Stefan; Reimann, Andreas; Fischer, Rainer

Spiegel, Holger; Schinkel, Helga; Kastilan, Robin; Dahm, Pia; Boes, Alexander; Scheuermayer, Matthias; Chudobová, Ivana; Maskus, Dominika; Fendel, Rolf; Schillberg, Stefan; Reimann, Andreas; Fischer, Rainer.

Affiliation

Spiegel H; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, Aachen, 52074, Germany. holger.spiegel@ime.fraunhofer.de.

Biotechnol Bioeng ; 112(4): 659-67, 2015 Apr.

Article in En | MEDLINE | ID: mdl-25335451

Subject(s)

Malaria Vaccines/biosynthesis; Malaria Vaccines/isolation & purification; Peptide Hydrolases/metabolism; Animals; Antibodies, Protozoan/blood; Binding Sites; Biotechnology/methods; Fluorescent Antibody Technique, Direct; Immunization/methods; Immunoglobulin G/blood; Malaria Vaccines/chemistry; Malaria Vaccines/genetics; Mass Spectrometry; Mice; Mutant Proteins/biosynthesis; Mutant Proteins/chemistry; Mutant Proteins/genetics; Mutant Proteins/isolation & purification; Pichia/genetics; Pichia/metabolism; Plasmodium falciparum/immunology; Proteolysis; Rabbits; Sequence Deletion; Technology, Pharmaceutical/methods; Vaccines, Synthetic/biosynthesis; Vaccines, Synthetic/chemistry; Vaccines, Synthetic/genetics; Vaccines, Synthetic/isolation & purification

Key words

Pichia pastoris; Plasmodium falciparum; protein engineering; proteolytic degradation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Hydrolases / Malaria Vaccines Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Biotechnol Bioeng Year: 2015 Document type: Article Affiliation country: Germany Country of publication: United States

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