ß-Casein(94-123)-derived peptides differently modulate production of mucins in intestinal goblet cells.
J Dairy Res
; 82(1): 36-46, 2015 Feb.
Article
in En
| MEDLINE
| ID: mdl-25335546
ABSTRACT
We recently reported the identification of a peptide from yoghurts with promising potential for intestinal health the sequence (94-123) of bovine ß-casein. This peptide, composed of 30 amino acid residues, maintains intestinal homoeostasis through production of the secreted mucin MUC2 and of the transmembrane-associated mucin MUC4. Our study aimed to search for the minimal sequence responsible for the biological activity of ß-CN(94-123) by using several strategies based on (i) known bioactive peptides encrypted in ß-CN(94-123), (ii) in silico prediction of peptides reactivity and (iii) digestion of ß-CN(94-123) by enzymes of intestinal brush border membranes. The revealed sequences were tested in vitro on human intestinal mucus-producing HT29-MTX cells. We demonstrated that ß-CN(108-113) (an ACE-inhibitory peptide) and ß-CN(114-119) (an opioid peptide named neocasomorphin-6) up-regulated MUC4 expression whereas levels of the secreted mucins MUC2 and MUC5AC remained unchanged. The digestion of ß-CN(94-123) by intestinal enzymes showed that the peptides ß-CN(94-108) and ß-CN(117-123) were present throughout 1·5 to 3 h of digestion, respectively. These two peptides raised MUC5AC expression while ß-CN(117-123) also induced a decrease in the level of MUC2 mRNA and protein. In addition, this inhibitory effect was reproduced in airway epithelial cells. In conclusion, ß-CN(94-123) is a multifunctional molecule but only the sequence of 30 amino acids has a stimulating effect on the production of MUC2, a crucial factor of intestinal protection.
Key words
ACE Angiotensin-converting enzyme; BBMV brush border membrane vesicles; BSA bovine serum albumin; CT controls; DPP IV dipeptidyl peptidase IV; ELLA Enzyme-linked lectin assay; FBS foetal bovine serum; Milk bioactive peptides; TFA trifluoroacetic acid; intestinal barrier; intestinal protection; mucus; ß-CN ß-casein; ß-casein
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
Caseins
/
Goblet Cells
/
Intestines
/
Mucins
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
J Dairy Res
Year:
2015
Document type:
Article
Affiliation country:
France