Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force.
Science
; 346(6209): 1254211, 2014 Oct 31.
Article
in En
| MEDLINE
| ID: mdl-25359979
ABSTRACT
Linkage between the adherens junction (AJ) and the actin cytoskeleton is required for tissue development and homeostasis. In vivo findings indicated that the AJ proteins E-cadherin, ß-catenin, and the filamentous (F)-actin binding protein αE-catenin form a minimal cadherin-catenin complex that binds directly to F-actin. Biochemical studies challenged this model because the purified cadherin-catenin complex does not bind F-actin in solution. Here, we reconciled this difference. Using an optical trap-based assay, we showed that the minimal cadherin-catenin complex formed stable bonds with an actin filament under force. Bond dissociation kinetics can be explained by a catch-bond model in which force shifts the bond from a weakly to a strongly bound state. These results may explain how the cadherin-catenin complex transduces mechanical forces at cell-cell junctions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actin Cytoskeleton
/
Cadherins
/
Actins
/
Adherens Junctions
/
Mechanotransduction, Cellular
/
Catenins
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Science
Year:
2014
Document type:
Article
Affiliation country:
United States