Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers.
Sensors (Basel)
; 14(11): 20519-32, 2014 Oct 30.
Article
in En
| MEDLINE
| ID: mdl-25360580
ABSTRACT
This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oximetry
/
Thermography
/
Biosensing Techniques
/
Tissue Array Analysis
/
Glucose
/
Neoplasms, Experimental
Limits:
Humans
Language:
En
Journal:
Sensors (Basel)
Year:
2014
Document type:
Article
Affiliation country:
United kingdom