Osthole induces apoptosis, suppresses cell-cycle progression and proliferation of cancer cells.
Anticancer Res
; 34(11): 6473-80, 2014 Nov.
Article
in En
| MEDLINE
| ID: mdl-25368248
ABSTRACT
BACKGROUND:
The aim of the present study was to determine the effects of osthole on cell proliferation and viability, cell-cycle progression and induction of apoptosis in human laryngeal cancer RK33 and human medulloblastoma TE671 cell lines. MATERIALS ANDMETHODS:
Cell viability was measured by means of the MTT method and cell proliferation by the 5-bromo-2-deoxyuridine (BrdU) incorporation assay. Cell-cycle progression was determined by flow cytometry, and induction of apoptosis by release of oligonucleosomes to the cytosol. The gene expression was estimated by a quantitative polymerase chain reaction (qPCR) method. High-performance counter-current chromatography (HPCCC) was applied for isolation of osthole from fruits of Mutellina purpurea.RESULTS:
Osthole decreased proliferation and cell viability of cancer cells in a dose-dependent manner. The tested compound induced apoptosis, increased the cell numbers in G1 and decreased cell number in S/G2 phases of the cell cycle, differentially regulating CDKN1A and TP53 gene expression depending on cancer cell type.CONCLUSION:
Osthole could be considered as a potential compound for cancer therapy and chemoprevention.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Cycle
/
Cerebellar Neoplasms
/
Laryngeal Neoplasms
/
Apoptosis
/
Coumarins
/
Cell Proliferation
/
Medulloblastoma
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
En
Journal:
Anticancer Res
Year:
2014
Document type:
Article
Affiliation country:
Poland