A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.
Soft Matter
; 11(2): 414-21, 2015 Jan 14.
Article
in En
| MEDLINE
| ID: mdl-25408475
ABSTRACT
The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended ß-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Hydrogel, Polyethylene Glycol Dimethacrylate
/
Seminal Vesicle Secretory Proteins
Limits:
Humans
Language:
En
Journal:
Soft Matter
Year:
2015
Document type:
Article
Affiliation country:
Sweden