Editing plant genomes with CRISPR/Cas9.
Curr Opin Biotechnol
; 32: 76-84, 2015 Apr.
Article
in En
| MEDLINE
| ID: mdl-25437637
ABSTRACT
CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants
/
Genome, Plant
/
Clustered Regularly Interspaced Short Palindromic Repeats
Limits:
Animals
/
Humans
Language:
En
Journal:
Curr Opin Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
United kingdom
Country of publication:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM