Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay.

da Silva, Cassia Maria Lins; Spinelli, Eliani; Rodrigues, Silvana Vianna

da Silva, Cassia Maria Lins; Spinelli, Eliani; Rodrigues, Silvana Vianna.

Affiliation

da Silva CM; Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista S/N, Centro, Niterói, RJ 24020-150, Brazil.
Spinelli E; Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mario Viana 523, Niterói, RJ 24241-000, Brazil.
Rodrigues SV; Instituto de Química, Universidade Federal Fluminense, Outeiro de São João Batista S/N, Centro, Niterói, RJ 24020-150, Brazil. Electronic address: silvana@vm.uff.br.

Food Chem ; 173: 619-23, 2015 Apr 15.

Article in En | MEDLINE | ID: mdl-25466067

Subject(s)

Collagen/analysis; Hydroxyproline/analysis; Meat Products/analysis; Animals; Biological Assay; Colorimetry/methods; Fasting; Hydrogen-Ion Concentration; Hydrolysis; Liver/chemistry; Sensitivity and Specificity; Sodium Hydroxide; Solutions; Spectrophotometry/methods; Swine

Key words

Alkaline hydrolysis; Collagen; Hydroxyproline; Protein; Spectrophotometry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Collagen / Hydroxyproline / Meat Products Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Food Chem Year: 2015 Document type: Article Affiliation country: Brazil

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