Nanotemplated polyelectrolyte films as porous biomolecular delivery systems. Application to the growth factor BMP-2.
Biomatter
; 4: e28823, 2014.
Article
in En
| MEDLINE
| ID: mdl-25482416
ABSTRACT
Biomaterials capable of delivering controlled quantities of bioactive agents, while maintaining mechanical integrity, are needed for a variety of cell contacting applications. We describe here a nanotemplating strategy toward porous, polyelectrolyte-based thin films capable of controlled biomolecular loading and release. Films are formed via the layer-by-layer assembly of charged polymers and nanoparticles (NP), then chemically cross-linked to increase mechanical rigidity and stability, and finally exposed to tetrahydrofuran to dissolve the NP and create an intra-film porous network. We report here on the loading and release of the growth factor bone morphogenetic protein 2 (BMP-2), and the influence of BMP-2 loaded films on contacting murine C2C12 myoblasts. We observe nanotemplating to enable stable BMP-2 loading throughout the thickness of the film, and find the nanotemplated film to exhibit comparable cell adhesion, and enhanced cell differentiation, compared with a non-porous cross-linked film (where BMP-2 loading is mainly confined to the film surface).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Biocompatible Materials
/
Myoblasts
/
Electrolytes
/
Bone Morphogenetic Protein 2
Limits:
Animals
Language:
En
Journal:
Biomatter
Year:
2014
Document type:
Article
Affiliation country:
France