Attached and planktonic Listeria monocytogenes global proteomic responses and associated influence of strain genetics and temperature.
J Proteome Res
; 14(2): 1161-73, 2015 Feb 06.
Article
in En
| MEDLINE
| ID: mdl-25516393
ABSTRACT
Contamination of industrial and domestic food usage environments by the attachement of bacterial food-borne pathogen Listeria monocytogenes has public health and economic implications. Comprehensive proteomics experiments using label-free liquid chromatography/tandem mass spectrometry were used to compare the proteomes of two different L. monocytogenes strains (Siliken_1/2c and F2365_4b), which show very different capacities to attach to surfaces. Growth temperature and strain type were highly influential on the proteomes in both attached and planktonic cells. On the basis of the proteomic data, it is highly unlikely that specific surface proteins play a direct role in adherence to inanimate surfaces. Instead, strain-dependent responses related to cell envelope polymer biosynthesis and stress response regulation likely contribute to a different ability to attach and also to survive external stressors. Collectively, the divergent proteome-level responses observed define strain- and growth-temperature-dependent differences relevant to attachment efficacy, highlight relevant proteins involved in stress protection in attached cells, and suggest that strain differences and growth conditions are important in relation to environmental persistence.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Temperature
/
Bacterial Proteins
/
Proteomics
/
Listeria monocytogenes
Type of study:
Risk_factors_studies
Language:
En
Journal:
J Proteome Res
Journal subject:
BIOQUIMICA
Year:
2015
Document type:
Article
Affiliation country:
Brazil