Glucocorticoid induced autophagy in N1511 chondrocyte cells.
Eur Rev Med Pharmacol Sci
; 18(23): 3573-9, 2014.
Article
in En
| MEDLINE
| ID: mdl-25535125
ABSTRACT
OBJECTIVE:
To determine whether autophagy was involved in chondrocyte cells post Glucocorticoids (GCs) treatment. MATERIALS ANDMETHODS:
LC3-GFP reporter plasmid transfection and western blotting analysis were conducted to determine the autophagic vesicles and autophagy-associated molecules in the N1511 chondrocyte cells post dexamethasone (Dex) treatment. And the N1511 cell viability was also determined by MTT assay.RESULTS:
We found that autophagy was induced in the N1511 chondrocyte cells post treatment with Dex of 5 µM to 1 mM, and the autophagy-induction by Dex could be inhibited by 3 MA and RU486, a GC antagonist. And the autophagy induced by the high dose of Dex (200 µM or 1 mM) was associated with a reduction of N1511 cell viability.CONCLUSIONS:
These results suggested that GCs could induce autophagy, as might contribute to the viability reduction of chondrocyte cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy
/
Chondrocytes
/
Glucocorticoids
Limits:
Animals
Language:
En
Journal:
Eur Rev Med Pharmacol Sci
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2014
Document type:
Article
Affiliation country:
China
Publication country:
IT
/
ITALIA
/
ITALY
/
ITÁLIA