Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method.
Bioanalysis
; 7(2): 207-20, 2015.
Article
in En
| MEDLINE
| ID: mdl-25587837
ABSTRACT
BACKGROUND:
In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS &METHODOLOGY:
Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrilemethanol (9010 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD<7.0 ng ml(-1)), linear (r>0.99), precise (RSD<11.3%), accurate (RE<11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®.CONCLUSION:
The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzophenones
/
Blood Chemical Analysis
/
Carbidopa
/
Dihydroxyphenylalanine
/
Dopamine
/
Levodopa
/
Catechols
/
Chromatography, High Pressure Liquid
/
Tandem Mass Spectrometry
/
Nitriles
Limits:
Humans
Language:
En
Journal:
Bioanalysis
Year:
2015
Document type:
Article
Affiliation country:
Brazil