The conjugative DNA-transfer apparatus of Streptomyces.
Int J Med Microbiol
; 305(2): 224-9, 2015 Feb.
Article
in En
| MEDLINE
| ID: mdl-25592263
ABSTRACT
Conjugation is a major route of horizontal gene transfer, an important driving force in the evolution of bacterial genomes. Since antibiotic producing streptomycetes represent a natural reservoir of antibiotic resistance genes, the Streptomyces conjugation system might have a particular role in the dissemination of the resistance genes. Streptomycetes transfer DNA in a unique process, clearly distinguished from the well-known DNA-transfer by type IV secretion systems. A single plasmid-encoded DNA-translocase, TraB, transfers a double-stranded DNA-molecule to the recipient. Elucidation of the structure, pore forming ability and DNA binding characteristics of TraB indicated that the TraB conjugation system is derived from an FtsK-like ancestor protein suggesting that Streptomyces adapted the FtsK/SpoIIIE chromosome segregation system to transfer DNA between two distinct Streptomyces cells. Following the primary transfer, a multi-protein DNA-translocation apparatus consisting of TraB and several Spd-proteins spreads the newly transferred DNA to the neighbouring mycelial compartments resulting in the rapid colonization of the recipient mycelium by the donor DNA.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptomyces
/
Bacterial Proteins
/
DNA, Bacterial
/
Conjugation, Genetic
/
Gene Transfer, Horizontal
Language:
En
Journal:
Int J Med Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Germany