Aquaporin-5 is expressed in adipocytes with implications in adipose differentiation.
IUBMB Life
; 67(1): 54-60, 2015 Jan.
Article
in En
| MEDLINE
| ID: mdl-25631586
ABSTRACT
Aquaporins (AQPs) are membrane channels widely distributed in nature. Typically, multiple isoforms are expressed in a single tissue. The adipose tissue is no exception where several AQP members have been identified. The importance of overlapped AQPs expression is unclear, yet interisoforms interactions might be required for key cellular functions. Recently, AQP5 was described as a regulator of other AQP isoforms. Therefore, we hypothesized for a role of AQP5 in adipocyte biology. Gene expression analysis revealed the presence of AQP5 in both 3T3-L1 fibroblasts and adipocytes, being more abundant in the later. AQP5 depletion impaired adipocyte differentiation, which was confirmed by decreased expression of specific differentiation markers. By overexpressing the human AQP5 in mature adipocytes it was possible to ascertain its role as a water channel in a gain-of-function scenario. To our knowledge, this is the first time that AQP5 is reported on adipose tissue. Our data revealed AQP5 as a new player in adipose tissue biology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Adipocytes
/
Aquaporin 5
Limits:
Animals
/
Humans
Language:
En
Journal:
IUBMB Life
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2015
Document type:
Article
Affiliation country:
Portugal