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Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties.
Nordin, Joel Z; Lee, Yi; Vader, Pieter; Mäger, Imre; Johansson, Henrik J; Heusermann, Wolf; Wiklander, Oscar P B; Hällbrink, Mattias; Seow, Yiqi; Bultema, Jarred J; Gilthorpe, Jonathan; Davies, Tim; Fairchild, Paul J; Gabrielsson, Susanne; Meisner-Kober, Nicole C; Lehtiö, Janne; Smith, C I Edvard; Wood, Matthew J A; El Andaloussi, Samir.
Affiliation
  • Nordin JZ; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Lee Y; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom.
  • Vader P; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom.
  • Mäger I; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom; Institute of Technology, University of Tartu, Tartu, Estonia.
  • Johansson HJ; Cancer Proteomics Mass Spectrometry, Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
  • Heusermann W; Novartis Institutes for Biomedical Research, Basel, Switzerland.
  • Wiklander OP; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Hällbrink M; Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
  • Seow Y; Molecular Engineering Laboratory, Proteos, Agency for Science, Technology and Research (A*STAR), Singapore.
  • Bultema JJ; Department of Medicine Solna, Translational Immunology Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.
  • Gilthorpe J; Umeå Centre for Molecular Medicine, Umeå University, Umeå, Sweden.
  • Davies T; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
  • Fairchild PJ; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
  • Gabrielsson S; Department of Medicine Solna, Translational Immunology Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden.
  • Meisner-Kober NC; Novartis Institutes for Biomedical Research, Basel, Switzerland.
  • Lehtiö J; Cancer Proteomics Mass Spectrometry, Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
  • Smith CI; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Wood MJ; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom. Electronic address: matthew.wood@dpag.ox.ac.uk.
  • El Andaloussi S; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom. Electronic address: Samir.El-Andaloussi@ki.se.
Nanomedicine ; 11(4): 879-83, 2015 May.
Article in En | MEDLINE | ID: mdl-25659648
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell-Derived Microparticles Type of study: Guideline Limits: Humans Language: En Journal: Nanomedicine Journal subject: BIOTECNOLOGIA Year: 2015 Document type: Article Affiliation country: Sweden Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell-Derived Microparticles Type of study: Guideline Limits: Humans Language: En Journal: Nanomedicine Journal subject: BIOTECNOLOGIA Year: 2015 Document type: Article Affiliation country: Sweden Country of publication: United States