Isolation and Identification of Brucella melitensis Biovar 3 from Vaccinated Small Ruminants: A Public Health Threat in Kosovo.
Transbound Emerg Dis
; 63(6): e296-e299, 2016 Dec.
Article
in En
| MEDLINE
| ID: mdl-25704160
ABSTRACT
In 2011, a human brucellosis case with severe clinical symptoms was reported at the University Clinic for Infectious Diseases in Prishtina, Kosovo. A trace-back investigation was conducted to find the source of human infection. A total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised on the patient's farm were taken for serological and molecular analysis. Serology using RBT and CFT revealed 11 positive animals. Twelve milk samples were PCR positive. A Brucella strain isolated from a goat's milk sample was classified as Brucella melitensis biovar 3, indicating the first ever isolation and report in Kosovo. The use of the Bruce-ladder PCR provided differentiation between the field strain and the vaccine strain. Hence, the accidental transmission of the vaccine strain Rev 1 that was previously used for the vaccination of the farm animals could be excluded. The findings of this study show that brucellosis is still a public health threat in Kosovo despite control measures.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ruminants
/
Brucellosis
/
Zoonoses
/
Public Health
/
Brucella melitensis
/
Lung Diseases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Aspects:
Determinantes_sociais_saude
Limits:
Animals
/
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
Transbound Emerg Dis
Journal subject:
MEDICINA VETERINARIA
Year:
2016
Document type:
Article
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY