Induction of PERV antigen in porcine peripheral blood mononuclear cells by human herpesvirus 1.
Xenotransplantation
; 22(2): 144-50, 2015.
Article
in En
| MEDLINE
| ID: mdl-25716804
ABSTRACT
BACKGROUND:
Xenotransplantation represents one of alternative candidates for allotransplantation due to the chronic shortage of suitable human tissues; however, many obstacles remain. Expression and release of endogenous retroviral antigens by porcine cells after transplantation may evoke adverse immune responses in human subjects. Here, we examined whether human herpesvirus 1 (HHV-1) could induce the production of porcine endogenous retrovirus (PERV) antigens in porcine peripheral blood mononuclear cells (PBMCs).METHODS:
Porcine PBMCs were infected with HHV-1 and examined for the production of PERV Gag protein and HHV-1 using antigen-capture ELISA and quantitative real-time polymerase chain reaction (PCR), respectively.RESULTS:
HHV-1 infection resulted in a 1.7- to 33.2-fold induction of PERV Gag relative to mock infection controls, compared to a 2.9- to 12.9-fold induction following treatment with PMA. Expression of PERV Gag was detected in porcine PBMCs and PK-15 cells after HHV-1 infection by double immunofluorescence staining of PERV Gag and HHV-1 antigen. The viability of HHV-1-infected porcine PBMCs was significantly lower than that of mock-infected cells. The HHV-1 level in the culture supernatant increased 5.2-fold relative to controls 24-h post-infection, indicative of active replication within these cells; decreased levels of HHV-1 were detected 72-h post-infection.CONCLUSIONS:
These results suggest that HHV-1 may be capable of infecting transplanted porcine cells, resulting in strong direct induction of PERV antigen.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
Leukocytes, Mononuclear
/
Herpesvirus 1, Human
/
Endogenous Retroviruses
/
Heterografts
/
Antigens, Viral
Limits:
Animals
/
Humans
Language:
En
Journal:
Xenotransplantation
Journal subject:
TRANSPLANTE
Year:
2015
Document type:
Article