Method for observing phase objects without halos or directional shadows.
Opt Lett
; 40(5): 812-5, 2015 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-25723439
ABSTRACT
A new microscopy method for observing phase objects without halos or directional shadows is proposed. The method is based on transformation of the surface profile of phase objects into a light intensity pattern. The key optical element is an annular aperture at the front focal plane of a condenser. The light flux passing through the annular aperture is changed by the specimen's surface profile and then passes through an objective and contributes to image formation. Theory and experimental results are presented. Phase images of induced pluripotent stem (iPS) cells with clear outlines were obtained with this method.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microscopy, Phase-Contrast
/
Artifacts
Limits:
Animals
Language:
En
Journal:
Opt Lett
Year:
2015
Document type:
Article