Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.

Hong, Seok Hoon; Kwon, Yong-Chan; Martin, Rey W; Des Soye, Benjamin J; de Paz, Alexandra M; Swonger, Kirsten N; Ntai, Ioanna; Kelleher, Neil L; Jewett, Michael C

Hong, Seok Hoon; Kwon, Yong-Chan; Martin, Rey W; Des Soye, Benjamin J; de Paz, Alexandra M; Swonger, Kirsten N; Ntai, Ioanna; Kelleher, Neil L; Jewett, Michael C.

Affiliation

Hong SH; Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Tech E-136, Evanston, IL 60208 (USA).

Chembiochem ; 16(5): 844-53, 2015 Mar 23.

Article in En | MEDLINE | ID: mdl-25737329

Subject(s)

Biotechnology; Escherichia coli/genetics; Escherichia coli/metabolism; Genetic Engineering; Peptide Termination Factors/deficiency; Protein Biosynthesis; Amino Acids/chemistry; Amino Acids/metabolism; Cell-Free System; Escherichia coli Proteins/genetics; Green Fluorescent Proteins/biosynthesis; Green Fluorescent Proteins/chemistry; Peptide Termination Factors/genetics

Key words

cell-free protein synthesis; genome engineering; non-standard amino acids; release factor 1; synthetic biology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Biosynthesis / Biotechnology / Genetic Engineering / Peptide Termination Factors / Escherichia coli Language: En Journal: Chembiochem Journal subject: BIOQUIMICA Year: 2015 Document type: Article Country of publication: Germany

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