Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.

Maruyama, Takeshi; Dougan, Stephanie K; Truttmann, Matthias C; Bilate, Angelina M; Ingram, Jessica R; Ploegh, Hidde L

Maruyama, Takeshi; Dougan, Stephanie K; Truttmann, Matthias C; Bilate, Angelina M; Ingram, Jessica R; Ploegh, Hidde L.

Affiliation

Maruyama T; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Dougan SK; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Truttmann MC; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Bilate AM; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Ingram JR; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Ploegh HL; 1] Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA. [2] Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Nat Biotechnol ; 33(5): 538-42, 2015 May.

Article in En | MEDLINE | ID: mdl-25798939

Subject(s)

CRISPR-Cas Systems/genetics; DNA End-Joining Repair/genetics; DNA Ligases/genetics; Genetic Engineering/methods; Animals; Cell Line; DNA Breaks, Double-Stranded/drug effects; DNA Ligase ATP; DNA Repair/genetics; Genome; Homologous Recombination/genetics; Mice; Pyrimidines/administration & dosage; Schiff Bases/administration & dosage; Zinc Fingers/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Ligases / Genetic Engineering / DNA End-Joining Repair / CRISPR-Cas Systems Limits: Animals Language: En Journal: Nat Biotechnol Journal subject: BIOTECNOLOGIA Year: 2015 Document type: Article Affiliation country: United States Country of publication: United States

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