C-peptide and zinc delivery to erythrocytes requires the presence of albumin: implications in diabetes explored with a 3D-printed fluidic device.
Integr Biol (Camb)
; 7(5): 534-43, 2015 May.
Article
in En
| MEDLINE
| ID: mdl-25825241
ABSTRACT
People with type 1 diabetes (T1D) must administer insulin exogenously due to the destruction of their pancreatic ß-cells. Endogenous insulin is stored in ß-cell granules along with C-peptide, a 31 amino acid peptide that is secreted from these granules in amounts equal to insulin. Exogenous co-administration of C-peptide with insulin has proven to reduce diabetes-associated complications in animals and humans. The exact mechanism of C-peptide's beneficial effects after secretion from the ß-cell granules is not completely understood, thus hindering its development as an exogenously administered hormone. Monitoring tissue-to-tissue communication using a 3D-printed microfluidic device revealed that zinc and C-peptide are being delivered to erythrocytes by albumin. Upon delivery, erythrocyte-derived ATP increased by >50%, as did endothelium-derived NO, which was measured downstream in the 3D-printed device. Our results suggest that hormone replacement therapy in diabetes may be improved by exogenous administration of a C-peptide ensemble that includes zinc and albumin.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zinc
/
C-Peptide
/
Diabetes Mellitus, Type 1
/
Albumins
/
Erythrocytes
/
Lab-On-A-Chip Devices
/
Printing, Three-Dimensional
Limits:
Animals
/
Humans
Language:
En
Journal:
Integr Biol (Camb)
Journal subject:
BIOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
United States