Ubiquitous L1 mosaicism in hippocampal neurons.
Cell
; 161(2): 228-39, 2015 Apr 09.
Article
in En
| MEDLINE
| ID: mdl-25860606
ABSTRACT
Somatic LINE-1 (L1) retrotransposition during neurogenesis is a potential source of genotypic variation among neurons. As a neurogenic niche, the hippocampus supports pronounced L1 activity. However, the basal parameters and biological impact of L1-driven mosaicism remain unclear. Here, we performed single-cell retrotransposon capture sequencing (RC-seq) on individual human hippocampal neurons and glia, as well as cortical neurons. An estimated 13.7 somatic L1 insertions occurred per hippocampal neuron and carried the sequence hallmarks of target-primed reverse transcription. Notably, hippocampal neuron L1 insertions were specifically enriched in transcribed neuronal stem cell enhancers and hippocampus genes, increasing their probability of functional relevance. In addition, bias against intronic L1 insertions sense oriented relative to their host gene was observed, perhaps indicating moderate selection against this configuration in vivo. These experiments demonstrate pervasive L1 mosaicism at genomic loci expressed in hippocampal neurons.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Long Interspersed Nucleotide Elements
/
Hippocampus
/
Mosaicism
/
Neurons
Limits:
Humans
Language:
En
Journal:
Cell
Year:
2015
Document type:
Article
Affiliation country:
Australia