Method for collecting and immobilizing individual cumulus cells enabling quantitative immunofluorescence analysis of proteins.

Appeltant, R; Maes, D; Van Soom, A

Appeltant, R; Maes, D; Van Soom, A.

Affiliation

Appeltant R; Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium. Electronic address: ruth.appeltant@ugent.be.
Maes D; Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
Van Soom A; Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.

Anal Biochem ; 480: 31-3, 2015 Jul 01.

Article in En | MEDLINE | ID: mdl-25862082

Subject(s)

Cumulus Cells/chemistry; Disintegrins/analysis; Fluorescent Antibody Technique; Matrix Metalloproteinases/analysis; Animals; Matrix Metalloproteinases/metabolism; Swine

Key words

Cell-Tak; Cumulus cells; Immobilization; Protein immunostaining; proADAMTS-1

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fluorescent Antibody Technique / Disintegrins / Matrix Metalloproteinases / Cumulus Cells Limits: Animals Language: En Journal: Anal Biochem Year: 2015 Document type: Article Country of publication: United States

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