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Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth.
Xie, Y M; Jin, L; Chen, X J; He, M N; Wang, Y; Liu, R; Li, M Z; Li, X W.
Affiliation
  • Xie YM; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Jin L; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Chen XJ; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • He MN; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Wang Y; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Liu R; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Li MZ; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Li XW; Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China xuewei.li@sicau.edu.cn.
Genet Mol Res ; 14(1): 1662-70, 2015 Mar 06.
Article in En | MEDLINE | ID: mdl-25867308
ABSTRACT
Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult. Observable differences in mtDNA content were detected in the tissues, including a 6-fold greater mtDNA content in the heart compared with the lung of 180-day-old samples. mtDNA content in the heart, longissimus dorsi muscle, psoas major muscle, kidney, brain, ovary, and subcutaneous adipose increased with growth. Expression of the replicative mitochondrial helicase (TWINKLE), which regulates mtDNA turnover, was significantly associated with changes in mtDNA copy numbers across tissues during growth (r = 0.33, P = 0.01). We demonstrated that the expression levels of mitochondrial genes were positively correlated with mtDNA copy number.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Swine / DNA, Mitochondrial / DNA Copy Number Variations Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country: China
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Swine / DNA, Mitochondrial / DNA Copy Number Variations Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country: China