[Effect of Photodynamic Inactivation (PDI) using Riboflavin-Conjugated Antibody against Staphylococcus aureus]. / Effekt photodynamischer Inaktivierung (PDI) mit Riboflavin oder riboflavinkonjugierten Antikörpern gegen Staphylococcus aureus.
Klin Monbl Augenheilkd
; 232(8): 988-92, 2015 Aug.
Article
in De
| MEDLINE
| ID: mdl-25927173
ABSTRACT
PURPOSE:
Crosslinking/riboflavin-UVA photodynamic therapy is a potential treatment alternative in antibiotic resistant infectious keratitis. For photodynamic therapy a specific (against bacteria) conjugated antibody may be used in order to increase the effect of the treatment. In our present study we analysed the impact of photodynamic inactivation using riboflavin-conjugated antibody or riboflavin alone on Staphylococcus aureus, in vitro.METHODS:
Staphylococcus aureus (S. aureus) was incubated in 1ââ100 diluted riboflavin-conjugated antibody (R-AB) for 30 minutes in darkness. Following UVA-light illumination (375 nm) with an energy dose of 2, 3, 4 and 8 J/cm(2), bacteria were brought to blood agar Plates for 24 hours before colony-forming unit (CFU) counting. In an additional group, we incubated bacteria to 0, 0.05 or 0.1â% riboflavin 5-phosphate as described above followed by illumination using UVA light (375 nm) with an energy dose of 2 J/cm(2), before CFU counting.RESULTS:
The number of CFU decreased significantly (inactivation of 36â%, p = 0.022) using 1ââ100 diluted riboflavin-conjugated antibody and 2 J/cm(2) UVA-light illumination, compared to untreated controls. The use of 3, 4 und 8 J/cm(2) energy dose and R-AB in 1ââ100 dilution did not further change the decrease of CFU (inactivation of 39, 39 and 40â%; p = 0.016; p = 0.016; p = 0.015). The use of 0.05â% or 0.1â% riboflavin 5-phosphate alone and UVA-light illumination reduced the CFU count significantly (inactivation of 73 and 55â%; p = 0.002; p = 0.005), compared to untreated controls.CONCLUSIONS:
The use of riboflavin-conjugated antibody or 0.05â% or 0.1â% riboflavin 5-phosphate and UVA-light illumination reduces the number of CFU of S. aureus. However, none of these photodynamic therapies reached the necessary 99â% killing rate of these bacteria. Further work is needed to increase the efficacy of riboflavin-conjugated antibodies against antibiotic resistant bacteria.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Photochemotherapy
/
Riboflavin
/
Staphylococcus aureus
/
Ultrasonic Therapy
/
Disinfection
Language:
De
Journal:
Klin Monbl Augenheilkd
Year:
2015
Document type:
Article
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY